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Journal: Nature Communications
Article Title: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice
doi: 10.1038/s41467-025-59985-8
Figure Lengend Snippet: a Ligand activities (left, brown), potential receptors (middle, red), and predicted target genes (right, blue) identified by NicheNet analysis based on the key signature genes of transferred OT-II T cells in spleens from WT mice, at day 2.5 post-HDI of pHBV1.3/OVA323-339. Heatmaps showing the absolute expression levels of ligands, receptors, and target genes are also shown. b Representative Violet tracer dilution and proliferation index of OT-II T cells at day 3.5 in the spleens, dLNs, and livers from WT mice treated with anti-Itgb2/LFA1 antibody versus control antibodies, at day 3.5 post-HDI of pHBV1.3/OVA323-339 ( n = 6). c Percentages of dead cells within transferred OT-II T cells in spleens, dLNs, and livers from the mice described in ( b ) using the viability dye eFluor 780 ( n = 6). d Percentages and number of CXCR5 + Bcl6 hi cells within transferred OT-II T cells in spleens, dLNs, and livers from the mice described in ( b , n = 6). e Same as ( b ), but mice were treated with control or CTLA4-Ig protein ( n = 3). f Same as c , but mice were treated with control or CTLA4-Ig protein ( n = 3). g Same as ( d ), but mice were treated with control or CTLA4-Ig protein ( n = 3). At least two independent experiments were performed. Horizontal lines indicate median values. Statistical significance was calculated using two-tailed Student’s t -tests. Significance levels are indicated as: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Source data and exact p -values are available in the Source Data file.
Article Snippet: For competitive blocking of ALCAM-CD6 and CD80-CD28 pathways, 10 μg/ml
Techniques: Expressing, Control, Two Tailed Test